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Regul Pept. 2009 Oct 7;
Tang SS, Du MH, Zhang JH, Kong X, Liu JJ Growth hormone releasing hormone (GHRH) is one of the hypothalamus hormones. For its potential applications in agriculture and medicine, GHRH analog with higher activity and longer half-life has been looked for. By using the fusion expression with unique acid labile linker Asp-Pro and biochemical purification, the three novel GHRH peptides, Pro-Pro-hGHRH(1-44)-Gly-Gly-Cys, Pro-hGHRH(1-44)-Gly-Gly-Cys, and (1)Pro-GHRH(2-44)-Gly-Gly-Cys, were obtained. The peptide molecular weight with 5,455, 5,373, or 5,210Da measured by EIS-MS is coincident with the actual values. The peptides at 0.1-10mug/ml increased rat pituitary GH releases in a dose-dependent manner and at 5mug/ml increased human pituitary GH releases. The activity comparisons showed that at 10mug/ml there were significant between (1)Pro-hGHRH(2-44)-Gly-Gly-Cys and Pro-Pro-hGHRH(1-44)-Gly-Gly-Cys or Pro-hGHRH(1-44)-Gly-Gly-Cys, (1)Pro-hGHRH(2-44) (P
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