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Old 12-11-2008, 10:05 PM
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rss Cre-mediated recombination in pituitary somatotropes.

pa href=http://www.hubmed.org/fulltext.cgi?uids=19039787Genesis/a. 2008 Nov 26; brNasonkin IO, Potok MA, Camper SApWe report a transgenic line with highly penetrant cre recombinase activity in the somatotrope cells of the anterior pituitary gland. Expression of the cre transgene is under the control of the locus control region of the human growth hormone gene cluster and the rat growth hormone promoter. Cre recombinase activity was assessed with two different lacZ reporter genes that require excision of a floxed stop sequence for expression: a chick beta-actin promoter with the CMV enhancer transgene and a ROSA26 knock-in. Cre activity is detectable in the developing pituitary after initiation of Gh transcription and persists through adulthood with high penetrance in Gh expressing cells and lower penetrance in lactotropes, a cell type that shares a common origin with somatotropes. This Gh-cre transgenic line is suitable for efficient, cell-specific deletion of floxed regions of genomic DNA in differentiated somatotropes and a subset of lactotrope cells of the anterior pituitary gland. genesis 0:1-6, 2008. (c) 2008 Wiley-Liss, Inc.

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