World Class Bodybuilding   Forum

BulkSupplements Egg Whites International A1Supplements.com i-Supplements HideMyAss
Go Back   World Class Bodybuilding Forum > WorldClass RSS Feeds > Peptides RSS FFEDS




Reply
 
Thread Tools Search this Thread Display Modes
  #1  
Old 04-20-2017, 01:00 PM
basskiller's Avatar
basskiller basskiller Is Off Line
Administrator

 
Join Date: Jan 2004
Location: Bodybuilding heaven
Posts: 117,466
Thanks: 3,195
Thanked 17,223 Times in 10,045 Posts
Rep Power: 2987
basskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond reputebasskiller has a reputation beyond repute
rss Human Growth Hormone - An Efficient and Rapid Method to Monitor the Oxidative Degrada

Related Articles An Efficient and Rapid Method to Monitor the Oxidative Degradation of Protein Pharmaceuticals: Probing Tyrosine Oxidation with Fluorogenic Derivatization.

Pharm Res. 2017 Apr 18;:

Authors: Bommana R, Mozziconacci O, John Wang Y, Schöneich C

Abstract
PURPOSE: The loss of potency of protein therapeutics can be linked to the oxidation of specific amino acid residues leading to a great variety of oxidative modifications. The comprehensive identification of these oxidative modifications requires high-resolution mass spectrometry analysis, which requires time and expensive resources. Here, we propose a fluorogenic derivatization method of oxidized Tyr and Phe yielding benzoxazole derivatives, as an orthogonal technique for the rapid screening of protein oxidation.
METHODS: Four model proteins, IgG1, human growth hormone (hGH), insulin and bovine serum albumin (BSA) were exposed to oxidation via peroxyl radicals and metal-catalyzed reactions and efficiently screened by fluorogenic derivatization of Tyr and Phe oxidation products. Complementary LC-MS analysis was done to identify the extent of methionine oxidation in oxidized proteins.
RESULTS: The Fluorogenic derivatization technique can easily be adapted to a 96-well plate, in which several protein formulations can be screened in short time. Representatively for hGH, we show that the formation of benzoxazole parallels the oxidation of Met to methionine sulfoxide which enables estimation of Met oxidation by just recording the fluorescence.
CONCLUSIONS: Our rapid fluorescence based screening allows for the fast comparison of the stability of multiple formulations.


PMID: 28421307 [PubMed - as supplied by publisher]



More...
Human growth hormone
HGH
GH
This is an automated post
__________________

W C B B Sponsors
Species Nutrition
Suppz.com - Bodybuilding Supplement Superstore

W C B B Affiliates
PowderCity.com
BulkSupplements.com
IslandSupplements.com
I-supplements.com
A1Supplements.com
HideMyAss Security Proxy<---- Protect your online activity
Home Chemistry Conversions
Egg Whites International <---100% Bio-Available Egg White Protein


Remember... Any advice given on this board is just an opinion and not to be taken as medical advice. WCBB doesn't advocate or condone use of steroids. Check the laws in your country. WCBB just provides the platform in which to discuss such matters
Reply With Quote
Get your liquid egg whites here at EGG WHITES INTERNATIONAL

Reply

Bookmarks


Currently Active Users Viewing This Thread: 2 (0 members and 2 guests)
 
Thread Tools Search this Thread
Search this Thread:

Advanced Search
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

Forum Jump


All times are GMT -4. The time now is 05:37 PM.



Copyright ©2000 - 2017, Jelsoft Enterprises Ltd.
follow worldclassbodybuilding at facebook follow worldclassbodybuilding at pinterest follow worldclassbodybuilding at twitter